- 1 What enzyme complexes break down protein?
- 2 Which of the following terms describes the DNA protein complexes?
- 3 What is the function of a spliceosome quizlet?
- 4 What is the name for the operator promoter and the genes they control?
- 5 What is the main function of a promoter?
- 6 Are arranged sequentially after the promoter?
- 7 What are the two methods of negative gene regulation?
- 8 What determines whether two proteins will bind to each other?
- 9 Which best describes a promoter?
- 10 What is the function of tRNA?
- 11 Which describes a promoter protein synthesis?
- 12 What prevents Lac genes?
- 13 What is the function of the lacZ gene?
- 14 What happens when the lac operon is turned off?
- 15 What is the lac operon an example of?
- 16 What is lac Z?
- 17 Is lac operon present in humans?
- 18 Is lac operon positive or negative?
- 19 What are 2 examples of proteins involved in positive regulation?
- 20 What is positive and negative control?
- 21 How is the lac operon controlled?
- 22 Is mutation lac operon?
- 23 What are the three important features of the lac operon?
- 24 Why does lac operon shut down?
- 25 Is lac operon active?
- 26 Why is low level of lac operon always required?
- 27 What is the lac operon and why is it important?
- 28 What happens to e coli When lactose is not present?
- 29 How is E coli lac operon switched on?
- 30 Which enzyme will be produced in a cell?
What enzyme complexes break down protein?
Proteins that are tagged for destruction are recognized and degraded by a complex of enzymes called the proteasome in the cytosol (cytosol = cytoplasm minus organelles) The proteasome has many proteases (enzymes that break down proteins). See Figure 8.42 in text.
Which of the following terms describes the DNA protein complexes?
Nucleosome: A section of DNA which is wrapped around proteins is called chromosomes. It forms a complex structure with proteins in the nucleus, which is called chromatin. The chromatin allows the DNA to be condensed into a smaller volume.
What is the function of a spliceosome quizlet?
What is the function of a “spliceosome”? The spliceosome splices out the non-coding introns from the primary mRNA transcript, and stitches the exons back together into the mature mRNA transcript.
What is the name for the operator promoter and the genes they control?
What is the main function of a promoter?
A promoter is a sequence of DNA needed to turn a gene on or off. The process of transcription is initiated at the promoter. Usually found near the beginning of a gene, the promoter has a binding site for the enzyme used to make a messenger RNA (mRNA) molecule.
Are arranged sequentially after the promoter?
The genes of an operon is/are arranged sequentially after the promoter. A(n) promoter is a specific nucleotide sequence in DNA that binds RNA polymerase, positioning it to start transcribing RNA at the appropriate place. In bacteria, this protein binds to the DNA in or near the promoter.
What are the two methods of negative gene regulation?
Negative gene regulation is when the operon is switched off by the active form of your repressor protein. The two types of proteins are repressor proteins and inducible proteins. Inducible proteins require the lac operon and the repressor proteins require the TRP operon. All proteins have the same three components.
What determines whether two proteins will bind to each other?
They will bind to each other if they have complementary shapes and chemical properties. A protein’s specific structure determines how it works, and in almost every case, the function of a protein depends on its ability to recognize and bind to some other molecule.
Which best describes a promoter?
Solution: The promoter is a nontranscribed region of a gene. The promoter is the regulatory region of a protein-coding gene at which RNA polymerase must bind to initiate transcription—it is not transcribed into the RNA.
What is the function of tRNA?
Transfer ribonucleic acid (tRNA) is a type of RNA molecule that helps decode a messenger RNA (mRNA) sequence into a protein. tRNAs function at specific sites in the ribosome during translation, which is a process that synthesizes a protein from an mRNA molecule.
Which describes a promoter protein synthesis?
Which describes a promoter? the first part of an operon that regulates where, when, and to what level a gene is expressed. Which is composed of amino acids and determines all the structures and functions of organisms? protein.
What prevents Lac genes?
The correct answer would be lac repressor. Lac repressor refers to the DNA binding protein which inhibits or prevents the expression of genes in the DNA of Lac Operon. The lac repressor protein is expressed by the Lac regulatory region.
What is the function of the lacZ gene?
lacZ encodes an enzyme that splits lactose into monosaccharides (single-unit sugars) that can be fed into glycolysis. Similarly, lacY encodes a membrane-embedded transporter that helps bring lactose into the cell.
What happens when the lac operon is turned off?
Normally, the lac operon is turned off. A repressor protein binds the operator (control) region upstream of the operon preventing transcription. When lactose is present outside the cell, it crosses the cell membrane and acts as an inducer of the operon. It does so once lactose is broken down to create allolactose.
What is the lac operon an example of?
The lac operon is a classic example an inducible operon. When lactose is present in the cell, it is converted to allolactose. Allolactose acts as an inducer, binding to the repressor and preventing the repressor from binding to the operator. This allows transcription of the structural genes.
What is lac Z?
The gene product of lacZ is β-galactosidase which cleaves lactose, a disaccharide, into glucose and galactose. lacY encodes Beta-galactoside permease, a membrane protein which becomes embedded in the cytoplasmic membrane to enable the cellular transport of lactose into the cell.
Is lac operon present in humans?
Operons are common in bacteria, but they are rare in eukaryotes such as humans. In general, an operon will contain genes that function in the same process. For instance, a well-studied operon called the lac operon contains genes that encode proteins involved in uptake and metabolism of a particular sugar, lactose.
Is lac operon positive or negative?
The lac operon is a negatively controlled inducible operon, where the inducer molecule is allolactose. In negative repressible operons, transcription of the operon normally takes place. Repressor proteins are produced by a regulator gene, but they are unable to bind to the operator in their normal conformation.
What are 2 examples of proteins involved in positive regulation?
In prokaryotes, a well-known activator protein is the catabolite activator protein (CAP), involved in positive control of the lac operon. In the regulation of gene expression, studied in evolutionary developmental biology (evo-devo), both activators and repressors play important roles.
What is positive and negative control?
A negative control is a control group in an experiment that uses a treatment that isn’t expected to produce results. A positive control is a control group in an experiment that uses a treatment that is known to produce results.
How is the lac operon controlled?
Regulation of the lac Operon The activity of the promoter that controls the expression of the lac operon is regulated by two different proteins. One of the proteins prevents the RNA polymerase from transcribing (negative control), the other enhances the binding of RNA polymerase to the promoter (positive control).
Is mutation lac operon?
Single mutants of the lac operon Such mutant are called constitutive mutants. Another type of mutant of lacI called Is prevents the repressor polypeptide from binding lactose, and thus will bind to the operator and be non-inducible.. This mutant constitutively represses the lac operon whether lactose is present or not.
What are the three important features of the lac operon?
The lac operon consists of three structural genes: lacZ, which codes for β-galactosidase, which acts to cleave lactose into galactose and glucose; lacY, which codes for lac permease, which is a transmembrane protein necessary for lactose uptake; and lacA, which codes for a transacetylase that transfers an acetyl group …
Why does lac operon shut down?
∗ Lactose is the inducer that inactivates repressor, after addition of lactose, β- galactosidese breaks lactose to galactose and glucose. Therefore, there is no more lactose to bind to the repressor protein and the lac operon shuts down.
Is lac operon active?
The lac operon contains an operator, promoter, and structural genes that are transcribed together and are under the control of the catabolite activator protein (CAP) or repressor. The lac operon is not activated and transcription remains off when the level of glucose is low or non-existent, but lactose is absent.
Why is low level of lac operon always required?
A low level of lac operon occurs due to the absence of formation of permeases. Permeases are necessary for the transport of lactose from medium into cells. Due to the failure of transport of lactose into the cell, it will not act as inducer.
What is the lac operon and why is it important?
The classic example of prokaryotic gene regulation is that of the lac operon. This operon is a genetic unit that produces the enzymes necessary for the digestion of lactose (Fig. 16-13). The lac operon consists of three contiguous structural genes that are transcribed as continuous mRNA by RNA polymerase.
What happens to e coli When lactose is not present?
What happens to E. coli when lactose is not present? The genes that produce the enzymes needed to break down lactose are not expressed. The repressor protein blocks the genes from making mRNA.
How is E coli lac operon switched on?
coli, the lac operon gets switched on when. lactose is present and it binds to RNA polymerase.
Which enzyme will be produced in a cell?
The gene Z is transcribed before Lac Y and A gene so due to nonsense mutation in the lac Y gene, a stop codon will appear in the sequence after lac Z gene. Thus β-galactosidase will be produced but no other enzyme will be produced.